Journal: Molecules

Article Title: Phenolic and Non-Polar Fractions of the Extracts from Fruits, Leaves, and Twigs of Elaeagnus rhamnoides (L.) A. Nelson—The Implications for Human Barrier Cells

doi: 10.3390/molecules25092238

Figure Lengend Snippet: Figure 3. IL-8 production by human foreskin fibroblasts line HFF-1 exposed to fractionated E. rhamnoides (L.) extracts and reference compounds: (a) 6 h; (b) 24 h, tested by ELISA. The results are given as a mean concentration [pg/mL] ± standard error. C1—control cells in culture medium with Me-OH, OF/GF/LF—phenolic fractions of fruit/twig/leaf extracts, C2—control cells in culture medium with DMSO, OL/GL/LL—non-polar fractions of fruit/twig/leaf extracts, PG—S. aureus peptidoglycan, TNF—murine tumor necrosis factor alpha, KU—ursolic acid, Q—quercetin, E—(−)-epicatechin, KE—ellagic acid; * p < 0.05, ** p < 0.01.

Article Snippet: Human epithelial colorectal adenocarcinoma cells (Caco-2, HTB-37) and human foreskin fibroblasts (HFF-1, SCRC-1041) were obtained from LGC Standards (Teddington, Middlesex, UK).

Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Control

Journal: Antioxidants

Article Title: Anthocyanin-Related Pigments: Natural Allies for Skin Health Maintenance and Protection

doi: 10.3390/antiox10071038

Figure Lengend Snippet: Cell viability of HaCat and HFF-1 cell lines in the presence of increasing concentrations of the compounds, evaluated by MTT assay. Dotted lines represent 80% viability.

Article Snippet: Spontaneously transformed aneuploid immortal keratinocyte cell lines from adult human skin (HaCat) and human foreskin fibroblasts (HFF-1) were cultured in 22.1 cm 2 plates and maintained in Dulbecco’s Modified Eagle Medium (DMEM, Cell Lines Service), supplemented with 10% or 15% fetal bovine serum (FBS, CLS) and 1% of antibiotic/antimycotic solution (100 units/mL of penicillin, 10 mg/mL of streptomycin and 0.25 mg/mL of amphotericin B, Sigma-Aldrich, St. Louis, MO, USA), at 37 °C in an atmosphere of 5% CO 2 .

Techniques: MTT Assay

Journal: Antioxidants

Article Title: Anthocyanin-Related Pigments: Natural Allies for Skin Health Maintenance and Protection

doi: 10.3390/antiox10071038

Figure Lengend Snippet: Effect of anthocyanins and related structures on ROS production in HaCat and HFF-1 cell lines, 24 h after incubation with 50 µM of each compound. Hydrogen peroxide (50 µM) was used as a positive control. Statistically significant differences between ROS production in the presence of the different compounds and control group are marked with asterisks: p < 0.05 (*), p < 0.005 (**) and p < 0.0001 (****). Dotted lines represent 80% ROS production.

Article Snippet: Spontaneously transformed aneuploid immortal keratinocyte cell lines from adult human skin (HaCat) and human foreskin fibroblasts (HFF-1) were cultured in 22.1 cm 2 plates and maintained in Dulbecco’s Modified Eagle Medium (DMEM, Cell Lines Service), supplemented with 10% or 15% fetal bovine serum (FBS, CLS) and 1% of antibiotic/antimycotic solution (100 units/mL of penicillin, 10 mg/mL of streptomycin and 0.25 mg/mL of amphotericin B, Sigma-Aldrich, St. Louis, MO, USA), at 37 °C in an atmosphere of 5% CO 2 .

Techniques: Incubation, Positive Control

Journal: AIDS Research and Human Retroviruses

Article Title: Purging Exhausted Virus-Specific CD8 T Cell Phenotypes by Somatic Cell Reprogramming

doi: 10.1089/aid.2017.0161

Figure Lengend Snippet: Establishment of B6/129OKSM mouse line. (A) Schematic illustration of B6/129OKSM mouse generation. We first established a DOX-reprogrammable fibroblast-like cell line originated from AinV15 mouse ESCs by transduction of STEM-CCA lentiviral vector encoding four DOX-inducible mouse reprogramming genes (Oct4, Klf4, Sox2, and c-Myc). Chimeric mice were generated by the injection of 8–12 established iPSCs into a B6 mouse blastocyst. The mice were bred with B6 mice to obtain F1 hybrids derived from 129P2/OlaHsd strain (agouti coat). F1 hybrids were then crossbred for >10 generations until we established a stable mouse line carrying a single copy of a reprogramming gene cassette introduced by STEM-CCA lentiviral vector homozygously and enabling induction of ESC-like colony formation from peripheral blood cells with high efficiency in the presence of DOX. DOX: doxycycline. Diff.: differentiation. (B) Fibroblasts were isolated from tail-tip biopsies of chimeric mice and exposed to 1 μg/ml of DOX for 2° iPSC induction. No 2° iPSC formation was seen with no DOX addition. (C) Expression of two pluripotent stem cell markers (Nanog and SSEA1) in 2° iPSCs. (D) Embryoid bodies generated from 2° iPSCs. iPSC, induced pluripotent stem cell.

Article Snippet: AinV15 mouse embryonic stem cells (ESCs) were purchased from American Type Culture Collection (ATCC SCRC-1029, Manassas, VA).

Techniques: Transduction, Plasmid Preparation, Generated, Injection, Derivative Assay, Isolation, Expressing